and Dr. Igawa (Hiroshima Univ. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure to water to spermatozoa of some aquatic species. Amphibian Research Center) through collaboration between the Xenopus Community in Japan (XCIJ) and National Bio-Resource Project (NBRP). Genetic analysis may be applicable to Xenopus (Silurana) tropicalis, which has a diploid genome and a s … Xenopus egg extract is a lysate that is prepared by crushing the eggs of the African clawed frog Xenopus laevis.It offers a powerful cell-free (or in vitro) system for studying various cell biological processes, including cell cycle progression, nuclear transport, DNA replication and chromosome segregation.It is also called Xenopus egg cell-free system or Xenopus egg cell-free extract. Garland Publishing Inc, New York ISBN 0-8153-1896-0. Xenopus contains at least three sarcomeric alpha actin genes that are preferentially expressed in either heart or skeletal muscle. Xenopus Tropicalis should be housed at 23-28 degrees C (75 -80 degrees F). The genome of X. tropicalis has been sequenced (Hellsten et al. These dual frog species boast experimental strengths for embryology including large egg sizes that develop externally, well‐defined fate maps, and cell‐intrinsic sources of nutrients that allow explanted tissues to grow in culture. Like Xenopus laevis, X. tropicalis was formerly used in pregnancy tests (Bergerard & Lamotte 1949). The African Clawed Frog (Xenopus laevis) is also known to tropical fish keeping enthusiasts as the Xenopus, African Clawed Toad, or African Claw Toed frog and is the only amphibian that actually has claws. X. laevis frogs are larger than X. tropicalis frogs and so are their cells, eggs, DNA content, and nuclei. A dye exclusion assay revealed that sperm plasma membrane integrity in both species decreased after freezing, more so for … A transgenic line of X. tropicalis was used to examine the process of cornea-lens transdifferentiation in which green fluorescent protein (GFP) is expressed in differentiated lens cells under the control of the Xenopus gamma1-crystallin promoter element. Genetic Map of Xenopus tropicalis Jamie Davies The kidney development database. The word Xenopus means "strange foot" and laevis means "smooth". We have demonstrated here that monospermy in the clawed frog, Xenopus tropicalis, as well as in X. laevis, is ensured by a fast, electrical block to polyspermy on the egg plasma membrane after the entry of the first sperm, which is mediated by the positive-going fertilization potential. Verlag der Zeitschrift für Naurforschung, Germany Google Scholar. Shook DR(1), Majer C, Keller R. Author information: (1)Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, VA 22903, USA. Xenopus laevis has long been a popular model for studies of development and, ... and genetically much more convenient diploid Xenopus tropicalis (Vogel, 1999). The early development of Xenopus Laevis, an atlas of the histology. Life cycle, evolutionary position and regenerative potential of Xenopus. Room temperature (controlled automatically by building … Introduction. 2010). Xenopus Laevis should be housed at 20-23 degrees C (68-75 degrees F). Due to the tetraploid nature of Xenopus laevis, each of these three alpha actin genes is present in at least two copies. Room Environmental Parameters. Phosphatase which catalyzes the conversion of NADP(+) to NAD(+) and of NADPH to NADH (By similarity). *Significantly greater or less (p < 0.05) than the corresponding values for controls. Jump to: navigation, search. Xenopus tropicalis Taxonomy ID: 8364 (for references in articles please use NCBI:txid8364) current name. From the early 1700s to the present, Xenopus have been studied as animal … Xenopus laevis (African clawed frog) Status. amphibians, which include Xenopus laevis (X. laevis) and Xenopus tropicalis (X. tropicalis), are one such group of animals with high regenerative capacity (Dinsmore, 1991). Xenopus tropicalis (Gray, 1864) basionym: Silurana tropicalis Gray, 1864. homotypic synonym: Xenopus (Silurana) tropicalis. 1. The cardiac versus skeletal expression patterns of actins are probably sequence-dependent. Exhibits a high amplitude circadian rhythm with maximal levels in early evening (PubMed:12573214). Reviewed-Annotation score: -Experimental evidence at transcript level i. Download Xenopus Genomes; X. laevis 9.2 (J-Strain) on JBrowse; X. laevis 9.2 (J-Strain) on UCSC; X. laevis 1.0 (wildtype) on JBrowse; X. tropicalis 10.0 (Nigerian) on JBrowse; X. tropicalis 9.1 (Nigerian) on JBrowse; X. tropicalis 9.1 (Nigerian) on UCSC; Xenbase UCSC Track Hub; Veenstra Lab UCSC Track Hub; Other amphibian genomes on JBrowse; Expression. Introduction. Xenopus tropicalis (Gray, 1864) homotypic synonym: Xenopus (Silurana) tropicalis. (2016) have sequenced the genome of Xenopus laevis. Xenopus tropicalis has been developed as a model organism for developmental biology, ... Three ngn subfamilies (Ngnr1–3) have been isolated in both Xenopus laevis and Xenopus tropicalis (Nieber et al., 2009). Shows a small preference for NADPH over NADP(+) (By similarity). The two best-known species of this genus are Xenopus laevis and Xenopus tropicalis, which are studied as model organisms for developmental biology, cell biology, toxicology, neuroscience and for modelling human disease and birth defects. Xenopus tropicalis was somewhat less sensitive to 6‐AN, semicarbizide and atrazine when tested at 27 °C than at 23 °C. Likewise, X. tropicalis has been selected over X. laevis as the subject of a complete genome sequencing project for the same reason. This is the first amphibian genome to be sequenced, enabling new insights into vertebrate evolution and the last common ancestor of tetrapods. However, X. laevis has an allotetraploid genome precluding its use in forward genetic analysis. Experiments were repeated three times. Axeldb A database focussing on gene expression in the frog Xenopus laevis. Xenopus tropicalis Taxonomy ID: 8364 (for references in articles please use NCBI:txid8364) current name. al. firstname.lastname@example.org The mesoderm, comprising the tissues that come to lie entirely in the deep layer, … The African clawed frog Xenopus tropicalis has several characteristics that facilitate studies of developmental and reproductive toxicity. Xenopus tropicalis and Xenopus laevis environmental parameter standard operating procedure (Kroll lab) From Xenbase. The African clawed frog (Xenopus laevis, also known as the xenopus, African clawed toad, African claw-toed frog or the platanna) is a species of African aquatic frog of the family Pipidae.Its name is derived from the three short claws on each hind foot, which it uses to tear apart its food. It was produced by Dr. Fukui (Faculty of Science and Engineering, Chuo Univ.) Xenopus laevis tropicalis. Xenopus tropicalis, on the other hand, possesses a diploid genome and a short generation time, ... (C and D) in stage 55 Xenopus laevis and Xenopus tropicalis tadpoles. A small, fast‐breeding, diploid relative of the frog Xenopus laevis, Xenopus tropicalis, has recently been adopted for research in developmental genetics and functional genomics.X. XDB is a Web data catalogue of NIBB/NIG Xenopus laevis EST project. Available as frozen sperm, please allow 8 months for live animals. Function i. 26. Reference: EXRC. Xenopus laevis (XLA) is an allotetraploid species which appears to have undergone whole-genome duplication after the interspecific hybridization of 2 diploid species closely related to Silurana/Xenopus tropicalis (XTR). Xenopus laevis is a scavenger and eats living, dead, or dying arthropods and other pieces of organic waste. Embryos develop externally and tadpoles are transparent, features that facilitate experimental manipulation and post-factum analysis of animals (Robert and Ohta, 2009; Banach and Robert, 2017). Robust and efficient protocols for fertilization and early embryo care of Xenopus laevis and Xenopus tropicalis are essential for experimental success, as well as maintenance and propagation of precious animal stocks. Carruthersa, S., et. Garland Publishing Inc, New York ISBN 0-8153-1896-0. Probable transcription factor. Xenopi cannot raise their body temperature by producing metabolic heat. Procedures input by VGP link to Kroll lab Xenopus environmental procedure SOP document at Washington University animal facility: Modified format below: A. The frog Xenopus laevis has provided significant insights into developmental and cellular processes. tropicalis shares advantages of X. laevis as a classic embryologic system, but its simpler genome and shorter generation time make it more convenient for multigenerational genetic, genomic, and transgenic … Component of the circadian clock or downstream effector of clock function (PubMed:12573214). … They must rely on external heat sources. Pattern and morphogenesis of presumptive superficial mesoderm in two closely related species, Xenopus laevis and Xenopus tropicalis. The rapid growth of the National Xenopus Resource has required effective implementation and optimization of these protocols. 1. XenOrtho DB (Xenopus Ortholog DataBase) is a database in which ortholog genes of Xenopus tropicalis, Xenopus laevis and humans are listed. Xenopus commonly known as the clawed frog) is a genus of aquatic frogs native to sub-Saharan Africa.. Twenty species are known. Xenopus laevis (also called the African Clawed frog due to the sharp, black claws in the hind inner three toes) is an aquatic amphibian of the Order Anuran and the family Pipidae.Xenopus are a long-lived, aquatic amphibian commonly used in teaching classrooms and in research laboratories. Because X. tropicalis is diploid and X. laevis is tetraploid, important inferences can be made about genome evolution. Silurana tropicalis Gray, 1864. Ngn1 is expressed in the otic placode at early embryological stages (up to NF22), but with somewhat stronger expression in Xenopus tropicalis than in Xenopus laevis. Digitized images and developmental data from Nieuwkoop and Faber (1994) Normal Table of Xenopus laevis (Daudin). Kalt MR (1976) Morphology and kinetics of spermatogenesis in Xenopus laevis. If the water temperature is sub optimal... frogs will not eat and their metabolism and immune systems will be depressed. Description: (Xic-GFP) 4.7 kb X. tropicalis p27 Xic1 promoter driving GFP expression in the neural tube and the eye. It is found in Nigeria, and from Sudan to South Africa where they are plentiful in the rivers and ponds within the southeastern portion of Sub Saharan Africa. X. laevis is the species of frog most often used to generate egg extracts. Xenopus laevis tropicalis. However, another species of frog Xenopus tropicalis can also be a source. Xenopus laevis and Xenopus tropicalis have been used extensively in developmental and cell biology, biochemistry, functional genomics and immunology. It has a voracious appetite and attacks anything that passes in front of it. It uses extremely sensitive fingers, an acute sense of smell, and its lateral line systems to locate food. Xenopus laevis and Xenopus tropicalis have long been used to drive discovery in developmental, cell, and molecular biology. Session et al. Lateral line systems, usually found in fish, detect vibrations in the water. Pedro's BioMolecular Research Tools A Collection of WWW Links to Information and Services Useful to Molecular Biologists.